Collaboration is not a science. To be successful, one needs to be in a constant learning mode, sharing best practices with others, reflecting deeply on one's own methodologies and constantly striving to define impact. The following insights and reflections are offered to assist others as they strive to drive their missions forward through collaborations. The comments in this paper reflect only the views of the Connect U.S. fund staff, based on their collective experience working on collaborative projects. The field of collaboration deserves broader, deeper, and more rigorous analysis and review. We hope this paper will help spur interested funders to take up that task.